In Vitro Study of the Effect of Nanoparticles on DNA deletion/mutations
Department
Chemistry
Major
Chemistry (Emphasis in Biochemistry)
Research Advisor
Ma, Yinfa
Advisor's Department
Chemistry
Funding Source
UMR Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Nanoparticles are widely used in a variety of industries ranging from beauty care products to videotape production, and their influence is increasing. Nanoparticles are easily inhaled into the lungs due to their extremely small size (less than 100 nm). Once inside the lungs, their influence is compounded due to their natural tendency to form large aggregates, which allows them to remain in the lungs for a longer period of time. Some preliminary data have demonstrated that these nanoparticles were cytotoxic. However, the mechanism for the toxicity is not yet clear. To study one possible cytotoxicity mechanism of nanoparticles, DNA was incubated with varying concentrations of three different sized nanoparticles, Fe2O3, CeO2, and Ti O2. The DNA fragments were separated by capillary gel electrophoresis to determine the amount of DNA deletion/mutations caused by each.
Biography
Julie Breckenridge is a senior undergraduate student at the University of Missouri--Rolla, majoring in Chemistry. She is the daughter of Mark and Mary Rose Breckenridge and is from Little Rock, AR.
Research Category
Natural Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Presentation Date
12 Apr 2006, 1:00 pm
In Vitro Study of the Effect of Nanoparticles on DNA deletion/mutations
Nanoparticles are widely used in a variety of industries ranging from beauty care products to videotape production, and their influence is increasing. Nanoparticles are easily inhaled into the lungs due to their extremely small size (less than 100 nm). Once inside the lungs, their influence is compounded due to their natural tendency to form large aggregates, which allows them to remain in the lungs for a longer period of time. Some preliminary data have demonstrated that these nanoparticles were cytotoxic. However, the mechanism for the toxicity is not yet clear. To study one possible cytotoxicity mechanism of nanoparticles, DNA was incubated with varying concentrations of three different sized nanoparticles, Fe2O3, CeO2, and Ti O2. The DNA fragments were separated by capillary gel electrophoresis to determine the amount of DNA deletion/mutations caused by each.
