Abstract
Experimental data from a chromatographic system involving the adsorption of bovine serum albumin (BSA) onto porous anion-exchange adsorbent particles packed in a column are presented. The parameters that characterize the mass transfer mechanisms of intraparticle diffusion and convection are estimated by fitting the predictions of dynamic mathematical models describing adsorption in column systems having spherical perfusive and purely diffusive adsorbent Particles to the experimental breakthrough data obtained from the column adsorption system. Both linear and nonlinear expressions for the equilibrium isotherm are considered. The values of the transport parameters are estimated in the time domain for the nonlinear adsorption models and in the Laplace transform domain for the linear adsorption models. The capabilities of the different models to describe satisfactorily the dynamic behavior of the adsorption system are compared. The dynamic nonlinear adsorption model for purely diffusive particles is found to describe most appropriately the dynamic behavior of the experimental chromatographic system studied in this work.
Recommended Citation
G. A. Heeter and A. I. Liapis, "Model Discrimination and Estimation of the Intraparticle Mass Transfer Parameters for the Adsorption of Bovine Serum Albumin Onto Porous Adsorbent Particles by the Use of Experimental Frontal Analysis Data," Journal of Chromatography A, vol. 776, no. 1, pp. 3 - 13, Elsevier, Jul 1997.
The definitive version is available at https://doi.org/10.1016/S0021-9673(97)00439-1
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
Adsorption; Albumin; Frontal analysis; Intraparticle mass transfer; Mathematical models; Proteins
International Standard Serial Number (ISSN)
0021-9673
Document Type
Article - Conference proceedings
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
25 Jul 1997
PubMed ID
9286073
Comments
Alexander von Humboldt-Stiftung, Grant None