Solvent Based 3D Printing of Biopolymer/Bioactive Glass Composite and Hydrogel for Tissue Engineering Applications
Department
Biological Sciences
Major
Chemical Engineering
Research Advisor
Semon, Julie A.
Advisor's Department
Biological Sciences
Abstract
Three-dimensional (3D) bioprinting is an emerging technology in which scaffolding materials and cell-laden hydrogels may be deposited in a predetermined fashion to create 3D porous constructs. A major challenge in 3D bioprinting is the slow degradation of melt deposited biopolymer. In this research, we describe a new method for printing poly-caprolactone (PCL)/bioactive borate glass composite as a scaffolding material and Pluronic F127 hydrogel as a cell suspension medium. Bioactive borate glass was added to a mixture of PCL and organic solvent to make an extrudable paste using one syringe while hydrogel was extruded and deposited in between the PCL/borate glass filaments using a second syringe. The degradation of the PCL/borate glass composite scaffold with and without the presence of hydrogel was investigated by soaking the scaffold in minimum essential medium. The weight loss of the scaffold together with formation of a hydroxyapatite-like layer on the surface shows the excellent bioactivity of the scaffold.
Biography
Jakeb Baldridge is graduating in May of 2018 with a (bio)chemical engineering degree. He has done undergraduate research for two years under Dr. Semon, had an REU at Oklahoma State, and had an internship at Bayer CropScience.
Presentation Type
OURE Fellows Final Oral Presentation
Document Type
Presentation
Location
Turner Room
Presentation Date
17 Apr 2018, 9:00 am - 9:30 am
Solvent Based 3D Printing of Biopolymer/Bioactive Glass Composite and Hydrogel for Tissue Engineering Applications
Turner Room
Three-dimensional (3D) bioprinting is an emerging technology in which scaffolding materials and cell-laden hydrogels may be deposited in a predetermined fashion to create 3D porous constructs. A major challenge in 3D bioprinting is the slow degradation of melt deposited biopolymer. In this research, we describe a new method for printing poly-caprolactone (PCL)/bioactive borate glass composite as a scaffolding material and Pluronic F127 hydrogel as a cell suspension medium. Bioactive borate glass was added to a mixture of PCL and organic solvent to make an extrudable paste using one syringe while hydrogel was extruded and deposited in between the PCL/borate glass filaments using a second syringe. The degradation of the PCL/borate glass composite scaffold with and without the presence of hydrogel was investigated by soaking the scaffold in minimum essential medium. The weight loss of the scaffold together with formation of a hydroxyapatite-like layer on the surface shows the excellent bioactivity of the scaffold.
