Regulation of Actin Binding Proteins During Cytokinesis
Department
Civil, Architectural and Environmental Engineering
Major
Civil Engineering
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
OURE
Abstract
Cytokinesis is the process of cell separation that occurs at the end of mitosis and meiosis. Dr. Shannon's lab uses the budding yeast Saccharomyces cerevisiae as a model eukaryotic cell to study the coordination of cytokinesis with the end of chromosome segregation. This research focuses on the effect that mutations of the protein IQG1 have on actin binding during cytokinesis. Mutations that affect phosphorylation of IQG1 cause cytokinesis defects and change the timing of actin ring assembly. I have prepared yeast protein extracts and used immunoprecipitation to purify IQG1. I will perform actin binding and bundling assays to determine if the mutations affect the interaction of IQG1 with actin filaments. By conducting more research on IQG1 and the role it plays in cell division, we stand to gain a better understanding of how large of a role IQG1 has in cytokinesis.
Biography
Scott Grier is an undergraduate student dual majoring in Civil Engineering and Engineering Management at Missouri S&T. This is his first experience with biology research.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium
Presentation Date
17 Apr 2018, 9:00 am - 12:00 pm
Regulation of Actin Binding Proteins During Cytokinesis
Upper Atrium
Cytokinesis is the process of cell separation that occurs at the end of mitosis and meiosis. Dr. Shannon's lab uses the budding yeast Saccharomyces cerevisiae as a model eukaryotic cell to study the coordination of cytokinesis with the end of chromosome segregation. This research focuses on the effect that mutations of the protein IQG1 have on actin binding during cytokinesis. Mutations that affect phosphorylation of IQG1 cause cytokinesis defects and change the timing of actin ring assembly. I have prepared yeast protein extracts and used immunoprecipitation to purify IQG1. I will perform actin binding and bundling assays to determine if the mutations affect the interaction of IQG1 with actin filaments. By conducting more research on IQG1 and the role it plays in cell division, we stand to gain a better understanding of how large of a role IQG1 has in cytokinesis.
