Hemolymph Extraction in Drosophila Melanogaster
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Thimgan, Matthew S.
Advisor's Department
Biological Sciences
Funding Source
UM Research Board, Missouri; Missouri S&T OURE
Abstract
Extraction of hemolymph from the fly is done by using a device composed of rubber tubbing, luer-locks, and pipet tips involving air pressure, as well. The air pressure guides the fly through the rubber tubbing and into the pipet tip, head first. The luer-locks are adjusted to apply the air pressure to only the pipet tip. One the fly is lodged into the end of the pipet tip, the antenna of the fly is removed and a little bit of air pressure is applied to push the hemolymph out of hole created by removing the antenna. This method will standardize the extraction of hemolymph by taking out the human factor of applying pressure and allowing air pressure to do the work. To record the amount of hemolymph extracted, the hemolymph drop gets transferred to hydrated paraffin oil. A picture is taken of the hemolymph drop in the oil and a volume calculation is performed.
Biography
Sarah is a junior from Richland, MO. She is studying Biological Sciences with an emphasis of Pre-Med and a minor in Chemistry. On campus, Sarah is a Student Ambassador for the Admissions office, the President of Scrubs Pre-Health Group, an Honors Academy student, and she is currently working in Dr. Thimgan’s Lab.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium
Presentation Date
17 Apr 2018, 9:00 am - 12:00 pm
Hemolymph Extraction in Drosophila Melanogaster
Upper Atrium
Extraction of hemolymph from the fly is done by using a device composed of rubber tubbing, luer-locks, and pipet tips involving air pressure, as well. The air pressure guides the fly through the rubber tubbing and into the pipet tip, head first. The luer-locks are adjusted to apply the air pressure to only the pipet tip. One the fly is lodged into the end of the pipet tip, the antenna of the fly is removed and a little bit of air pressure is applied to push the hemolymph out of hole created by removing the antenna. This method will standardize the extraction of hemolymph by taking out the human factor of applying pressure and allowing air pressure to do the work. To record the amount of hemolymph extracted, the hemolymph drop gets transferred to hydrated paraffin oil. A picture is taken of the hemolymph drop in the oil and a volume calculation is performed.
