The Effects of Gallein on M3 Muscarinic Receptors
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Aronstam, Robert
Advisor's Department
Biological Sciences
Funding Source
cDNA Resource Center
Abstract
Gallein is a large cyclic carbon molecule with multiple functional groups. With no known medical use and few research articles, the only known action is that the drug has been found to effect G-protein subunits, specifically the GBγ subunit expressed in native rat olfactory receptors. I determined the effects of Gallein on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol. Gallein had a distinct inhibitory effect on store operated calcium (SOCE) that was induced by activation of the M3 receptors. No inhibition of IP3 receptor mediated calcium release was seen. With more testing, Gallein might prove to have a pharmacological benefit.
Biography
Alex is a junior in Biology. While getting his B.A., he is also pursuing a minor in Chemistry and Spanish. He is the Public Relations Officer for the pre-health organization, Scrubs. He has hopes to go to medical school after his senior year and become a M.D. in Ophthalmology.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
10 Apr 2012, 9:00 am - 11:45 am
The Effects of Gallein on M3 Muscarinic Receptors
Upper Atrium/Hallway
Gallein is a large cyclic carbon molecule with multiple functional groups. With no known medical use and few research articles, the only known action is that the drug has been found to effect G-protein subunits, specifically the GBγ subunit expressed in native rat olfactory receptors. I determined the effects of Gallein on calcium signaling underlying transmission mediated by human M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Receptor binding was determined in radiolabelled ligand binding assays; changes in intracellular calcium concentrations were determined using a fura-2 ratiometric imaging protocol. Gallein had a distinct inhibitory effect on store operated calcium (SOCE) that was induced by activation of the M3 receptors. No inhibition of IP3 receptor mediated calcium release was seen. With more testing, Gallein might prove to have a pharmacological benefit.
