Effects of Nitric Oxide on Store Operated Calcium Entry
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Aronstam, Robert
Advisor's Department
Biological Sciences
Funding Source
Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.
Biography
Katie is in her second year at Missouri S&T. She is from Steelville, MO and plans to attend a medical school after receiving her undergraduate degree in Biological Sciences with a minor in Chemistry and Theatre. Katie Payne is active in Alpha Psi Omega, the National Theatre Honors society. Katie Payne works as a research assistant at Dr. Aronstam’s cDNA lab and she volunteers at the PCRMC Emergency Department in her free time.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
10 Apr 2012, 9:00 am - 11:45 am
Effects of Nitric Oxide on Store Operated Calcium Entry
Upper Atrium/Hallway
S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.
Comments
Joint project with Megan Koerner