Effects of Nitric Oxide on Store Operated Calcium Entry

Presenter Information

Megan Koerner

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.

Biography

Megan is a senior from Eldon, MO. She plans to attend medical school after receiving her bachelor’s degree in Biological Sciences. Megan is an active member in the Biological Sciences honor society. Megan is employed as a department recruiter for the Biological Sciences Department. She also volunteers in Dr. Aronstam’s laboratory, conducting research on cellular signal transduction. Megan has been awarded the Access Missouri Scholarship and SMART Grant all four years of academic study.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

10 Apr 2012, 9:00 am - 11:45 am

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Apr 10th, 9:00 AM Apr 10th, 11:45 AM

Effects of Nitric Oxide on Store Operated Calcium Entry

Upper Atrium/Hallway

S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.