Effects of Nitric Oxide on Store Operated Calcium Entry
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Aronstam, Robert
Advisor's Department
Biological Sciences
Funding Source
Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.
Biography
Megan is a senior from Eldon, MO. She plans to attend medical school after receiving her bachelor’s degree in Biological Sciences. Megan is an active member in the Biological Sciences honor society. Megan is employed as a department recruiter for the Biological Sciences Department. She also volunteers in Dr. Aronstam’s laboratory, conducting research on cellular signal transduction. Megan has been awarded the Access Missouri Scholarship and SMART Grant all four years of academic study.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
10 Apr 2012, 9:00 am - 11:45 am
Effects of Nitric Oxide on Store Operated Calcium Entry
Upper Atrium/Hallway
S-nitrosoglutathion (SNG) is a well known smooth muscle relaxant and a nitric oxide donor when introduced into a cell. SNG has a very short half life so it must be synthesized before every experiment. It was made by using Glutathione (GSH) and Sodium Nitrate (NaNO2). We determined the effects of nitric oxide on calcium signaling by utilizing M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Cytotoxicity was determined using a dye reduction assay. Nitric oxide was found to have an EC50 at 10mM, and had an inhibitory effect on store operated calcium entry (SOCE). Cytosolic calcium concentrations were measured using a calcium sensitive fluorescent dye (fura-2AM) and a ratiometric imaging program.
