Department
Biological Sciences
Major
Biology
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
UMR Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Cytokinesis is the physical separation of the cytoplasm after mitosis that results in two daughter cells. This is accomplished by the use of a contractile ring composed primarily of actin and myosin. This project used gene knockout techniques in S. cerevisiae to determine the effect a gene has on cytokinesis. We used one-step PCR-mediated gene replacement to delete an uncharacterized open reading frame (ORF) that has been identified in a genomic screen as interacting with the cytokinesis protein Hof1. Deletion of this ORF, YGR250C, resulted in a cytokinesis defect. The gene deletion resulted in 12% of the cells exhibiting a cytokinesis defect compared to a wild type strain that exhibited a 2% cytokinesis defect. The protein encoded by YGR250C ORF has homology to RNA-binding proteins. Further studies will determine if Hof1 mRNA or protein levels are affected by deletion of YGR250C.
Biography
Amy is a senior at the University of Missouri-Rolla majoring in biology. On campus she is the president of the biology organization Helix and involved in Phi Sigma.
Research Category
Natural Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Havener Center, Carver-Turner Room
Presentation Date
11 April 2007, 1:00 pm - 3:00 pm
Identification of an Uncharacterized RNA-binding protein that affects Cytokinesis in Saccharomyces cerevisiae.
Havener Center, Carver-Turner Room
Cytokinesis is the physical separation of the cytoplasm after mitosis that results in two daughter cells. This is accomplished by the use of a contractile ring composed primarily of actin and myosin. This project used gene knockout techniques in S. cerevisiae to determine the effect a gene has on cytokinesis. We used one-step PCR-mediated gene replacement to delete an uncharacterized open reading frame (ORF) that has been identified in a genomic screen as interacting with the cytokinesis protein Hof1. Deletion of this ORF, YGR250C, resulted in a cytokinesis defect. The gene deletion resulted in 12% of the cells exhibiting a cytokinesis defect compared to a wild type strain that exhibited a 2% cytokinesis defect. The protein encoded by YGR250C ORF has homology to RNA-binding proteins. Further studies will determine if Hof1 mRNA or protein levels are affected by deletion of YGR250C.
