Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Frank, Ron
Advisor's Department
Biological Sciences
Funding Source
UMR Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Gene family research is an important area of genetics which can help to identify evolutionary lines. Starting with the FtSH gene family in Arabidopsis, a well-studied relative of a well-studied relative of Glycine max, with the ultimate goal of finding members of the gene family in G. max, computer programs were used to compare the genes in Arabidopsis to more closely related plants. These were then compared to G. max ESTs, which were assembled into contigs where ORFs were identified. The contigs were compared and the EST sources for the best contigs were identified. Overall, five members of the FtSH gene family were identified. Once these are identified, the next stage is possible, where the ESTs are ordered in vector form, transformed into bacteria, cultured and isolated. This allows them to be sequenced and compared to the contigs to see if a gene family was identified.
Biography
David is a junior at the university of Missouri-Roi/a majoring in biological sciences. He is the son of Rick and Phoebe Calcara from Olympia, WA. On campus he is a member of the swim team, where he is both an All-American and an Academic All-American. He is still undecided as to his future career.
Research Category
Natural Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Havener Center, Carver-Turner Room
Presentation Date
11 April 2007, 1:00 pm - 3:00 pm
FtSH Gene Family Identification
Havener Center, Carver-Turner Room
Gene family research is an important area of genetics which can help to identify evolutionary lines. Starting with the FtSH gene family in Arabidopsis, a well-studied relative of a well-studied relative of Glycine max, with the ultimate goal of finding members of the gene family in G. max, computer programs were used to compare the genes in Arabidopsis to more closely related plants. These were then compared to G. max ESTs, which were assembled into contigs where ORFs were identified. The contigs were compared and the EST sources for the best contigs were identified. Overall, five members of the FtSH gene family were identified. Once these are identified, the next stage is possible, where the ESTs are ordered in vector form, transformed into bacteria, cultured and isolated. This allows them to be sequenced and compared to the contigs to see if a gene family was identified.
