Investigating the Role of Microtubules in Budding Yeast Cytokinesis
Department
Biological Sciences
Major
Biology
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
Missouri Alliance for Minority Participation and UMR Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
People have always assumed that Microtubules (MT) are not required in Yeast cytokinesis, research will show if this is true. We will look at budding yeast cells, because they are easier to manipulate. Budding yeast is common yeast used in baking (“baker’s Yeast”) and brewing (“brewer’s yeast”). It is a popular model organism in the laboratory for many reasons. One reason is that budding yeast is a unicellular eukaryote whose cellular activities are much like ours than a prokaryote like E.coli. Like E. coli, it can be cultured easily, grow rapidly, and easily transformed with genes from other sources. In mammalian cells MT determine the position of cytokinesis. We want to know are MT required for yeast cytokinesis ? If so we will investigate what actomyosin ring components are affected by the lack of microtubules. Our goal is to find what will happen when eliminating MT from the cell. Will the cells cytokinese. Is the actin ring involved? Does myosin localize or contract? We will manipulate these cells to determine cytokinesis defects. Preliminary data indicate that MT may be required for cytokinesis in budding yeast
- Delta mad2 delta bub2 cells on benomyl plates (MT poision) chains were evident
- We expect that Delta Mad2 delta bub2 cells of MT’s. We will use microscopy to observe actin and myosin
Biography
Merkeb Teame is a sophomore attending the University of Missouri--Rolla majoring in Biology. On campus she is active in Student council STUCO, and Vice President of African Student Association ASA.
Research Category
Research Proposals
Presentation Type
Poster Presentation
Document Type
Poster
Presentation Date
12 Apr 2006, 9:00 am
Investigating the Role of Microtubules in Budding Yeast Cytokinesis
People have always assumed that Microtubules (MT) are not required in Yeast cytokinesis, research will show if this is true. We will look at budding yeast cells, because they are easier to manipulate. Budding yeast is common yeast used in baking (“baker’s Yeast”) and brewing (“brewer’s yeast”). It is a popular model organism in the laboratory for many reasons. One reason is that budding yeast is a unicellular eukaryote whose cellular activities are much like ours than a prokaryote like E.coli. Like E. coli, it can be cultured easily, grow rapidly, and easily transformed with genes from other sources. In mammalian cells MT determine the position of cytokinesis. We want to know are MT required for yeast cytokinesis ? If so we will investigate what actomyosin ring components are affected by the lack of microtubules. Our goal is to find what will happen when eliminating MT from the cell. Will the cells cytokinese. Is the actin ring involved? Does myosin localize or contract? We will manipulate these cells to determine cytokinesis defects. Preliminary data indicate that MT may be required for cytokinesis in budding yeast
- Delta mad2 delta bub2 cells on benomyl plates (MT poision) chains were evident
- We expect that Delta Mad2 delta bub2 cells of MT’s. We will use microscopy to observe actin and myosin
