Masters Theses

Abstract

"Gene and protein therapeutics play critical roles in the drug market and represent the future of biopharmaceuticals. Polymeric nanoparticle-based gene/protein delivery systems are promising owing to their ability to protect the cargo from degradation, improving intracellular delivery and transfection efficiency as well as abundant sources and flexibility to be modified. Yet the successful industrialized production and clinical application of polymeric nanoparticle-based delivery systems have long been limited by unsatisfied delivery efficiency and the lack of reproducible and scalable methods for the preparation of uniform nanoparticles with good batch to batch consistency. To address the unsatisfied efficacy and difficulty in scale-up preparation of traditional gene and protein delivery systems, the goal of this project is to validate a scalable and reproducible fabrication technology that uses a water-based continuous nanoparticle generation platform (wNGP) for scalable production of uniform and highly efficient gene and protein delivery systems using functionalized polyamidoamine (PAMAM) dendrimers. The generation platform is a multi-inlet vortex mixer that was originally designed for flash nanoprecipitation. The feasibility of the application of this technology in the biopharmaceutical industry will be verified by the fabrication of a polymer/plasmid and polymer/protein nanocomplex using a functionalized PAMAM dendrimer and a model plasmid and protein. Key process parameters were identified to fabricate the nanocomplexes in a scalable way without sacrificing functionality"--Abstract, p. iii

Advisor(s)

Yang, Hu

Committee Member(s)

Wang, Jee-Ching
Convertine, Anthony

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

Missouri University of Science and Technology

Publication Date

Summer 2023

Pagination

ix, 40 pages

Note about bibliography

Includes_bibliographical_references_(pages 38-39)

Rights

© 2022 Joseph Timothy Johnston, All Rights Reserved

Document Type

Thesis - Open Access

File Type

text

Language

English

Thesis Number

T 12310

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