Masters Theses

Abstract

"The purpose of this investigation was to determine an assay method for measuring the activity of the indole acetic acid oxidase present in Hygrophorus conicus. This enzyme converts tryptamine or indole acetic acid (IAA) into oxindole acetic acid (OIAA).

An assay method for measuring the activity of the enzyme was developed based on the spectrophotometric measurement of tryptamine, IAA, and OIAA. Several other assay methods were tried. The colorimetric method of Gordon and Weber (l4) was found to be useful for a quantitative determination of IAA and was insensitive to OIAA. Paper chromatography proved to be useful as a qualitative estimation of OIAA.

The enzyme was found to be intracellular and synthesized only when the organism was grown in the presence of an inducer. The rate of induction of IAA oxidase in H. conicus was similar to that of inducible enzymes in other organisms"--Abstract, p. ii

Advisor(s)

Donald J, Siehr

Committee Member(s)

Frank H. Conrad, 1902-1983
Sotirios G. Grigoropoulos
Illegible Signature

Department(s)

Chemical and Biochemical Engineering

Degree Name

M.S. in Chemical Engineering

Publisher

University of Missouri at Rolla

Publication Date

1964

Pagination

vii, 63 pages

Note about bibliography

Includes bibliographical references (pages 59-61)

Rights

© 1964 Dilip N. Patel, All rights reserved.

Document Type

Thesis - Open Access

File Type

text

Language

English

Thesis Number

T 1555

Print OCLC #

5955306

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