Doctoral Dissertations

Keywords and Phrases

Legionella; Mass Spectrometry; Metabolomics; Traumatic Brain Injury

Abstract

"Pathological processes often involve complex biochemical changes which can be assessed using advanced mass spectrometry. In this present dissertation, two fields were studied: traumatic brain injury (TBI), and water contamination by L. pneumophilia. TBI is a pressing public health concern for which current clinical tools remain inadequate. We present newly developed mass spectrometric methods to access metabolites associated with blast induced TBI. We applied these methods to the biofluids of soldiers conducting explosives training. Significant changes in several metabolites were observed between pre- and post-blast specimens, including changes that increased with repeated exposure. These changes point to the possibility of a biomarker panel to assess the severity of blast related TBI. L. pneumophilia is a pathogenic bacteria which can infect domestic water supplies. Its control and prevention in these systems is of major public health consequence. We have developed a novel single cell-inductively coupled plasma- mass spectrometry (SC-ICP-MS) method and applied this method to investigate L. pneumophilia treatment efficiency by copper (Cu) in drinking water. We applied this method to L. pneumophilia dosed with Cu in varied concentrations and over a time. Interestingly, high concentrations of Cu ions were found to have a high disinfection rate in drinking water, some cells persisted and even returned to a normal state 24 hours after the initial exposure to Cu. These likely viable but non-culturable cells were detectable by SC-ICP-MS but not by colony forming unit count analyses. This is the first study to our knowledge which explores the relationship between copper dosing over time of L. pneumophilia in drinking water by SC-ICP-MS" -- Abstract, p. iv

Advisor(s)

Shi, Honglan
Nam, Paul Ki-souk
Burton, Casey

Committee Member(s)

Wang, Risheng
Westenberg, David J.

Department(s)

Chemistry

Degree Name

Ph. D. in Chemistry

Publisher

Missouri University of Science and Technology

Publication Date

Summer 2024

Pagination

xiii, 102 pages

Note about bibliography

Includes_bibliographical_references_(pages 29, 52, 85 & 91-101)

Rights

©2024 Austin Chase Sigler , All Rights Reserved

Document Type

Dissertation - Open Access

File Type

text

Language

English

Thesis Number

T 12430

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Chemistry Commons

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