Catalytic Method Determination of Serum Transferrin by Capillary Electrophoresis

Abstract

A high performance capillary electrophoresis (HPCE) catalytic method for the determination of serum transferrin concentration has been developed. The whole process includes techniques of pretreatment of serum samples and HPCE system optimization. For standard solution, a good linear response was maintained over the range 0. 5~10 µmol/L (r = 0. 995). Seven healthy adult serum samples were studied by this system and their transferrin concentrations are 23. 9, 25. 7, 26. 9, 24. 5, 23. 2, 25. 5 and 23. 7 µmol/L. 30 µL serum would be sufficient for transferrin measurement and the total serum pretreatment process takes less than 2 hours and the CE separation process takes only 6 min. This HPCE method is reliable, sensitive, rapid and efficient. Thus it is suitable for clinical application.

Department(s)

Chemistry

Keywords and Phrases

Capillary Electrophoresis; Catalytic Method; Iron; Serum; Transferrin

International Standard Serial Number (ISSN)

0253-3820

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

Chinese

Language 2

English

Rights

© 1998 Chinese Academy of Sciences, All rights reserved.

Publication Date

01 Jan 1998

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