Indirect Photometric Detection of Polyamines in Biological Samples Separated by High-Performance Capillary Eletrophoresis

Abstract

A rapid separation of polyamines and some related amino acids in cultured tumor cells by high-performance capillary zone electrophoresis with indirect photometric detection is demonstrated. 60 cm x 75 µm I.D. fused-silica capillary was used for the separation and quinine sulfate was used as a background electrolyte (BGE). Several polyamines (putrescine, spermidine and spermine), amino acids (lysine, arginine, histidine) and simple cations (K+, Na+) were easily separated in less than 10 min. Using the indirect photometric detection method, femtomole amounts of polyamines extracted from the tumor cells were detected from nanoliter injection volumes, and the signal response was linear over two orders of magnitude.

Department(s)

Chemistry

Keywords and Phrases

Polyamine; Electrophoresis; High Performance Liquid Chromatography; Photometry; Priority Journal; Zone Electrophoresis; Animal; Biogenic Polyamines; Electrolytes; Electrophoresis; Indicators And Reagents; PC12 Cells; Photometry; Rats; Support, Non-U.S. Gov't

International Standard Serial Number (ISSN)

0021-9673

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 1992 Elsevier, All rights reserved.

Publication Date

01 Sep 1992

Link to Full Text

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