Abstract
A vancomycin chiral stationary phase (CSP) was fully evaluated in capillary electrochromatography (CEC) in reversed phase and polar organic modes for a number of racemic pharmaceutical compounds. High efficiency and resolution values were obtained for a number of compound classes including thalidomide in both the polar organic mode (190 000 plates meter-1 and Rs = 13.8) and reversed-phase mode (125 000 plates meter-1 and Rs = 13.0). Experimental parameters, including organic modifier, organic solvent ratio, ionic strength, pH, temperature, and voltage, were examined in both the aqueous and nonaqueous modes to deduce their effect on the resultant EOF, retention times, resolution, and efficiency of chiral separations. All results were consistent with and found to be a combination of what is known from existing literature on CEC theory and experience obtained with macrocyclic antibiotic CSPs in LC. Column stability was excellent, and each column packed was found to offer repeatable separations even when switching from the aqueous to the nonaqueous mode.
Recommended Citation
C. Karlsson et al., "Evaluation of a Vancomycin Chiral Stationary Phase in Capillary Electrochromatography using Polar Organic and Reversed-phase Modes," Analytical Chemistry, vol. 72, no. 18, pp. 4394 - 4401, American Chemical Society, Sep 2000.
The definitive version is available at https://doi.org/10.1021/ac0002792
Department(s)
Chemistry
International Standard Serial Number (ISSN)
0003-2700
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 American Chemical Society, All rights reserved.
Publication Date
15 Sep 2000
PubMed ID
11008775
