Abstract
Enantiomeric separation of chiral pharmaceuticals is carried out in aqueous and non-aqueous packed capillary electrochromatography (CEC) using a teicoplanin chiral stationary phase (CSP). Capillaries were slurry packed with 5 μm 100-A porous silica particles modified with teicoplanin and initially evaluated using a non-aqueous polar organic mode system suitability test for the separation of metoprolol enantiomers (R(s)=2.3 and 53 000 plates m-1). A number of pharmaceutical drugs were subsequently screened with enantioselectivity obtained for 25 racemic solutes including examples of neutral, acidic and basic molecules such as coumachlor (R(s)=3.0 and 86 000 plates m-1) and alprenolol (R(s)=3.3 and 135 000 plates m-1) in reversed-phase and polar organic mode, respectively. A statistical experimental design was used to investigate the effects of non-aqueous polar organic mobile phase parameters on the CEC electroosmotic flow, resolution and peak efficiency for two model solutes. Results primarily indicated that higher efficiency and resolution values could be attained at higher methanol contents which is similar to findings obtained on this phase in liquid chromatography. Copyright (C) 2000 Elsevier Science B.V.
Recommended Citation
C. Karlsson et al., "Enantioselective Reversed-phase and Non-aqueous Capillary Electrochromatography using a Teicoplanin Chiral Stationary Phase," Journal of Chromatography A, vol. 897, no. 1 thru 2, pp. 349 - 363, Elsevier, Nov 2000.
The definitive version is available at https://doi.org/10.1016/S0021-9673(00)00805-0
Department(s)
Chemistry
Keywords and Phrases
Chiral stationary phases, CEC; Electrochromatography; Enantiomer separation; Experimental design; Teicoplanin
International Standard Serial Number (ISSN)
0021-9673
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
03 Nov 2000
PubMed ID
11128218
