Abstract
The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chiral stationary phase (CSP). Over 230 racemates were resolved in either the reversed-phase mode, the normal-phase mode, or the polar-organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed. The ristocetin A CSP appeared to be complimentary to other glycopeptide CSPs (i.e., vancomycin and teicoplanin). Column stability was excellent. The CSP was not irreversibly altered when going from one mobile phase mode to another.
Recommended Citation
K. H. Ekborg-Ott et al., "Highly Enantioselective HPLC Separations using the Covalently Bonded Macrocyclic Antibiotic, Ristocetin A, Chiral Stationary Phase," Chirality, vol. 10, no. 5, pp. 434 - 483, Wiley, Aug 1998.
The definitive version is available at https://doi.org/10.1002/(SICI)1520-636X(1998)10:5<434::AID-CHIR10>3.0.CO;2-0
Department(s)
Chemistry
Publication Status
Full Access
Keywords and Phrases
Chiral stationary phase; Enantiomeric separations; Macrocyclic antibiotic; Ristocetin A; Underivatized amino acids
International Standard Serial Number (ISSN)
0899-0042
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Wiley, All rights reserved.
Publication Date
08 Aug 1998
PubMed ID
9691460
Comments
National Institute of General Medical Sciences, Grant R01GM053825