Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells

Abstract

Previous studies have shown that chemotherapeutic efficacy could be enhanced with targeted drug delivery. Various DNA origami nanostructures have been investigated as drug carriers. Here, we compared drug delivery functionalities of three similar DNA origami nanostructures, Disc, Donut, and Sphere, that differ in structural dimension. Our results demonstrated that Donut was the most stable and exhibited the highest Dox-loading capacity. MUC1 aptamer modification in our nanostructures increased cellular uptake in MUC1-high MCF-7. Among the three nanostructures, unmodified Donut exerted the highest Dox cytotoxicity in MCF-7, and MUC1 aptamer modification did not further improve its effect, implicating that Dox delivery by Donut was efficient. However, all Dox-loaded nanostructures showed comparable cytotoxicity in MDA-MB-231 due to the innate sensitivity of this cell line to Dox. Our results successfully demonstrated that functional properties of DNA origami nanocarriers could be tuned by structural design, and three-dimensional Donut appeared to be the most efficient nanocarrier.

Department(s)

Chemistry

Comments

This work was financially supported by the Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation (Grant No. RGNS63-141), Burapha University (A.U.), and Mahidol University (T.K.).

Keywords and Phrases

DNA Origami Nanostructures; Doxorubicin; Drug Delivery System; Enhanced Specificity; MUC1 Aptamer

International Standard Serial Number (ISSN)

2576-6422

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2022 American Chemical Society, All rights reserved.

Publication Date

01 Jan 2022

PubMed ID

35500214

Link to Full Text

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