Evaluation of Hydroxyapatite Microspheres Made from a Borate Glass to Separate Protein Mixtures

Abstract

A hydroxyapatite (HA, Ca10(PO4)6(OH) 2), transformed from a calcium-containing borate glass, has been investigated for its protein adsorption and chromatographic characteristics. Microspheres of the borate glass were transformed into HA by reacting them with a 0.25 M phosphate (K2HPO4) solution for 24 h at 37°C (pH 9.0). The HA microspheres with a diameter of 45-90 µm were hand packed into a steel column (4.6 mm × 80 mm) and used to separate a binary protein mixture of bovine serum albumin (BSA) and lysozyme. HA microspheres, with a diameter <45 µm, were used for separating a protein mixture of BSA, myoglobin, and lysozyme. These microspheres had a diameter that was 20-30 times larger than commercial HA column packing spherical particles, 2-3 µm, but these microspheres had a six times larger surface area and a more uniform spherical shape. These advantages compensated for their larger size and the separation results were comparable to those commercially available HA columns in the separation of the proteins studied. These unique HA microspheres, made from microspheres of a borate glass, are considered to be useful as packing materials for protein separation in chromatography.

Department(s)

Chemistry

Second Department

Materials Science and Engineering

Sponsor(s)

University of Missouri--Rolla. Materials Research Center

Keywords and Phrases

Apatite; Calcium, Borate Glasses; Protein Mixtures, Hydroxyapatite; Protein - separation

International Standard Serial Number (ISSN)

0022-2461

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2008 Springer Verlag, All rights reserved.

Publication Date

01 Aug 2008

Link to Full Text

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