Abstract
The aim of the present study was to report on a complete synthetic approach, namely benzylation-hydrolysis-acylation-hydrogenation, to the synthesis of regioselectively acylated quercetin analogues using low-cost rutin as a starting material. Three quercetin analogues, quercetin-3-O-propionate (Q-pr), quercetin-3-O-butyrate (Q-bu) and quercetin-3-O-valerate (Q-va), containing 3-, 4- and 5-carbon aliphatic acyl chains, respectively, were synthesized and characterized with 1H nuclear magnetic resonance (NMR), 13C NMR and mass spectrometry. Compared with quercetin, all three analogues exhibited improved lipophilicity. The lipophilicity of the analogue increased with increasing acyl chain length. Q-va exhibited the highest lipophilicity among the three analogues, but a lower water solubility compared with quercetin. By contrast, Q-pr and Q-bu exhibited 8.2- and 4.7-fold higher water solubility compared with quercetin, respectively. The in vitro and in vivo studies demonstrated that Q-pr and Q-bu were more effective whereas Q-va was less effective in inhibiting platelet aggregation compared with quercetin. These results indicated that the water solubility and the lipophilicity of the analogues must be improved in order to achieve higher antiplatelet activity, and an optimal acyl chain length is crucial for the synthesized quercetin analogues to be more effective.
Recommended Citation
Y. Duan et al., "Synthesis of Regioselectively Acylated Quercetin Analogues with Improved Antiplatelet Activity," Molecular Medicine Reports, vol. 16, no. 6, pp. 9735 - 9740, Spandidos Publications, Dec 2017.
The definitive version is available at https://doi.org/10.3892/mmr.2017.7781
Department(s)
Chemical and Biochemical Engineering
Keywords and Phrases
Antiplatelet aggregation; Oil-water partition coefficient; Quercetin; Regioselective acylation; Water solubility
International Standard Serial Number (ISSN)
1791-2997; 1791-3004
Document Type
Article - Journal
Document Version
Final Version
File Type
text
Language(s)
English
Rights
© 2017 Spandidos Publications, All rights reserved.
Publication Date
01 Dec 2017
PubMed ID
29039540
Comments
The present study was supported by the Shandong Natural Science Foundation (grant no. ZR2010HQ052), the Medical and Health Science and Technology Development Project of Shandong Province (grant nos. 2011QZ025 and 2014WSB27002), the Pharmaceutical Technology Development Project of Shandong Province (grant no. 2013-238) and the Chinese Key Technology Program (grant no. 2013GA740103).