Abstract
With the aim of developing appropriate scaffolds for tissue engineering to suppress the formation of biofilms, an effective one-pot process was applied in this study to produce scaffolds with inherent antibacterial activity. A new method to synthesize genipin-crosslinked gelatin/nano silver scaffolds with "green" in situ formation of silver nanoparticles by heat treatment is presented in this paper. In this procedure, toxic solvents, reducing agents, and stabilizing agents are avoided. UV-visible absorption spectra of the synthesized gelatin/nano silver solutions were obtained immediately and three months after the synthesis revealing the presence and high stability of the silver nanoparticles. The TEM of gelatin/nano silver solutions showed silver particles with spherical shapes that were less than 5 nm in size. Interestingly, contact angle was found to increase from 80 to 125 with the increase in concentration of nano silver in gelatin. All gelatin/nano silver solutions showed antimicrobial activity against Staphylococcus aureus and Escherichia coli. However, only the highest concentration showed antifungal effects against Candida albicans pathogens. Scaffolds were prepared by a lyophilization technique from this solution and their antimicrobial activities were examined. Introducing this facile green one-pot process of synthesizing scaffolds with antimicrobial and anti-biofilm properties may lead to key applications in tissue engineering techniques. © 2014 Elsevier B.V. All rights reserved.
Recommended Citation
M. Yazdimamaghani and D. Vashaee and S. Assefa and M. Shabrangharehdasht and A. T. Rad and M. A. Eastman and K. J. Walker and S. V. Madihally and G. A. Köhler and L. Tayebi, "Green Synthesis Of A New Gelatin-based Antimicrobial Scaffold For Tissue Engineering," Materials Science and Engineering C, vol. 39, no. 1, pp. 235 - 244, Elsevier BV, Jun 2014.
The definitive version is available at https://doi.org/10.1016/j.msec.2014.03.007
Department(s)
Chemical and Biochemical Engineering
Publication Status
Complimentary Access
Keywords and Phrases
Antibacterial activity; Biofilm; Scaffold; Silver nanoparticle; Tissue engineering
International Standard Serial Number (ISSN)
0928-4931
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2025 Elsevier BV, All rights reserved.
Publication Date
01 Jun 2014
PubMed ID
24863221
