Hybrid Macroporous Gelatin/bioactive-glass/nanosilver Scaffolds With Controlled Degradation Behavior And Antimicrobial Activity For Bone Tissue Engineering

Abstract

A new composition of gelatin/bioactive-glass/silver nanoparticle was synthesized and employed to prepare antibacterial macroporous scaffolds with potential applications in bone tissue engineering. A set of macroporous nanocomposite scaffolds were developed from an aqueous solution of gelatin by freeze-drying and crosslinking using genipin at ambient temperature. Silver nanoparticles were successfully synthesized in situ in gelatin solution by heat treatment reduction as a simple and "green" method in which gelatin acted as a natural reducing and stabilizing agent. The effect of the incorporation of the bioactive-glass and the silver nanoparticle concentration on the physicochemical properties of the scaffolds, such as the gel fraction, porosity, in vitro enzyme degradation, morphology, and swelling behavior was studied. Furthermore, the in vitro viability of human mesenchymal stem cells (hMSC) and the antibacterial activity against gram-negative Escherichia coli and gram-positive Staphylococcus aureus were tested on the scaffolds. It was found that upon the addition of silver nanoparticles the porosity, pore size, swelling, and antibacterial properties were enhanced. The silver nanoparticles increased the in vitro enzyme degradation in samples without bioactive-glass; however, the degradation was remarkably reduced by addition of bioactive-glass. In addition, formation of apatite particles, the main inorganic constituent of the bone, on the surface of the bioactive-glass containing scaffolds were confirmed after immersion in simulated body fluid (SBF). The viability of hMSC on the scaffold suggested that gelatin/bioactive-glass/nanosilver scaffolds can be used as an antibacterial scaffolds. Copyright © 2014 American Scientific Publishers All rights reserved.

Department(s)

Chemical and Biochemical Engineering

Keywords and Phrases

Antibacterial; Bioactive-Glass; Bone; Gelatin; Green synthesis; Nanosilver; Scaffold; Tissue engineering

International Standard Serial Number (ISSN)

1550-7041; 1550-7033

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2025 American Scientific Publishers, All rights reserved.

Publication Date

01 Jan 2014

PubMed ID

24749388

Link to Full Text

Share

 
COinS