Time- And Dose-dependent Gene Expression Analysis Of Macrophage Response As A Function Of Porosity Of Silica Nanoparticles

Abstract

There is a limited amount of information available on gene expression regulation of macrophages in response to changing the time of exposure, concentration, and physicochemical properties of nanomaterials. In this study, RAW264.7 macrophages were treated with spherical nonporous and mesoporous silica nanoparticles of similar size at different incubation times and concentrations. RNA-sequencing was used to study transcriptional profiles. Bioinformatics analyses, functional annotation clustering, and network analyses were employed to understand signaling pathways of cellular response as a function of porosity, incubation time, and concentration. Porosity introduced drastic changes to the genomic response of macrophages at equitoxic concentrations and incubation times. Direct relations between increases in time and concentration with an increased number of differentially expressed genes were observed.

Department(s)

Chemical and Biochemical Engineering

Comments

National Institutes of Health, Grant P30 CA042014

Keywords and Phrases

Bioinformatics; Global gene expression; Network analysis; Signaling pathways; Silica nanoparticles

International Standard Serial Number (ISSN)

1549-9642; 1549-9634

Document Type

Article - Journal

Document Version

Citation

File Type

text

Language(s)

English

Rights

© 2025 Elsevier, All rights reserved.

Publication Date

01 Oct 2019

PubMed ID

31228603

Link to Full Text

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