Equilibrium and Kinetic Parameters of the Adsorption of Α-Chymotrypsinogen a Onto Hydrophobic Porous Adsorbent Particles
Abstract
Adsorption equilibria and rate kinetics for the binding of α-chymotrypsinogen A onto hydrophobic porous adsorbent particles, have been investigated for three different temperatures. The results show that the amount of protein adsorbed increases as the temperature increases. The values of the parameters that characterize the mechanisms of pore diffusion and adsorption were determined. The values of the pore diffusion coefficient and the values of the time constants for the mass transfer steps of pore diffusion and adsorption suggest that the pore diffusion mechanism in the porous structure of the adsorbent particles is rate limiting. An analysis of the results of the adsorption of α-chymotrypsinogen A suggests that the heat of adsorption is positive, and this would indicate that the adsorption of α-chymotrypsinogen A onto the hydrophobic adsorbent particles used in this work is entropically driven. © 1994.
Recommended Citation
A. Tongta et al., "Equilibrium and Kinetic Parameters of the Adsorption of Α-Chymotrypsinogen a Onto Hydrophobic Porous Adsorbent Particles," Journal of Chromatography A, vol. 686, no. 1, pp. 21 - 29, Elsevier, Nov 1994.
The definitive version is available at https://doi.org/10.1016/0021-9673(94)00066-2
Department(s)
Chemical and Biochemical Engineering
Second Department
Chemistry
International Standard Serial Number (ISSN)
0021-9673
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
25 Nov 1994
