Perfusion Chromatography. Effect of Micropore Diffusion on Column Performance in Systems Utilizing Perfusive Adsorbent Particles with a Bidisperse Porous Structure
Abstract
A mathematical model describing single-component and multi-component adsorption in columns with bidisperse perfusive or bidisperse purely diffusive adsorbent particles is constructed and presented. The model is used to study the adsorption of lysozyme onto monocional anti-lysozyme in columns with bidisperse porous adsorbent particles. The influence of the effective pore diffusion coefficient of the adsorbate in the microparticles (microspheres) and the effects of particle size and intraparticle convective flow on column performance are examined. The results for the systems studied indicate that the systems with bidisperse perfusive particles provide a higher dynamic utilization of the adsorptive capacity of the column than the systems having bidisperse purely diffusive particles. © 1994.
Recommended Citation
A. I. Liapis and M. A. McCoy, "Perfusion Chromatography. Effect of Micropore Diffusion on Column Performance in Systems Utilizing Perfusive Adsorbent Particles with a Bidisperse Porous Structure," Journal of Chromatography A, vol. 660, no. 1 thru 2, pp. 85 - 96, Elsevier, Feb 1994.
The definitive version is available at https://doi.org/10.1016/0021-9673(94)85102-6
Department(s)
Chemical and Biochemical Engineering
International Standard Serial Number (ISSN)
0021-9673
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2024 Elsevier, All rights reserved.
Publication Date
04 Feb 1994
Comments
North Atlantic Treaty Organization, Grant 880770