GPR183-oxysterol Axis In Spinal Cord Contributes To Neuropathic Pain
Abstract
Neuropathic pain is a debilitating public health concern for which novel non-narcotic therapeutic targets are desperately needed. Using unbiased transcriptomic screening of the dorsal horn spinal cord after nerve injury we have identified that Gpr183 (Epstein-Barr virus-induced gene 2) is upregulated after chronic constriction injury (CCI) in rats. GPR183 is a chemotactic receptor known for its role in the maturation of B cells, and the endogenous ligand is the oxysterol 7a,25-dihydrox-ycholesterol (7a,25-OHC). The role of GPR183 in the central nervous system is not well characterized, and its role in pain is unknown. The profile of commercially available probes for GPR183 limits their use as pharmacological tools to dissect the roles of this receptor in pathophysiological settings. Using in silico modeling, we have screened a library of 5 million compounds to identify several novel small-molecule antagonists of GPR183 with nanomolar potency. These compounds are able to antagonize 7a,25-OHC-induced calcium mobilization in vitro with IC50 values below 50 nM. In vivo intrathecal injections of these antagonists during peak pain after CCI surgery reversed allodynia in male and female mice. Acute intrathecal injection of the GPR183 ligand 7a,25-OHC in naïve mice induced dose-dependent allodynia. Importantly, this effect was blocked using our novel GPR183 antagonists, suggesting spinal GPR183 activation as pronociceptive. These studies are the first to reveal a role for GPR183 in neuropathic pain and identify this receptor as a potential target for therapeutic intervention. SIGNIFICANCE STATEMENT We have identified several novel GPR183 antagonists with nanomolar potency. Using these antagonists, we have demonstrated that GPR183 signaling in the spinal cord is pronociceptive. These studies are the first to reveal a role for GPR183 in neuropathic pain and identify it as a potential target for therapeutic intervention.
Recommended Citation
K. Braden and L. A. Giancotti and Z. Chen and C. DeLeon and N. Latzo and T. Boehm and N. D'Cunha and B. M. Thompson and T. M. Doyle and J. G. McDonald and J. K. Walker and G. R. Kolar and C. K. Arnatt and D. Salvemini, "GPR183-oxysterol Axis In Spinal Cord Contributes To Neuropathic Pain," Journal of Pharmacology and Experimental Therapeutics, vol. 375, no. 2, pp. 367 - 375, American Society for Pharmacology and Experimental Therapeutics (ASPET), Nov 2020.
The definitive version is available at https://doi.org/10.1124/JPET.120.000105
Department(s)
Biological Sciences
International Standard Serial Number (ISSN)
1521-0103; 0022-3565
Document Type
Article - Journal
Document Version
Citation
File Type
text
Language(s)
English
Rights
© 2025 American Society for Pharmacology and Experimental Therapeutics (ASPET), All rights reserved.
Publication Date
01 Nov 2020
PubMed ID
32913007
Comments
National Heart, Lung, and Blood Institute, Grant P01-HL020948