Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
FYRE
Abstract
During cytokinesis, the division of the cytoplasm between cells is controlled by the contraction of an actomyosin ring. Iqg1, a crucial regulator of cytokinesis in Saccharomyces cerevisiae, is needed for the assembly and contraction of this ring. Already, it has been found that a domain in the Iqg1 C-terminus is crucial for S. cerevisiae survival. In this study, a regulated Iqg1 allele is introduced to yeast. This will allow researchers to turn off the wild-type allele of the Iqg1 gene under specified conditions, which allows for the study of the effects of the C-terminal Iqg1 deletion. Progress towards making the desired yeast strain is monitored by making protein extracts to examine Iqg1 expression. This research into Iqg1 could potentially provide insight into public health, as IQGAP proteins, a family in which Iqg1 is a member, have implications in human cancer.
Biography
Rae Tardif/a is currently a B.S. Biological Sciences freshman at Missouri S& T. They are currently involved in iGem, Kummer Vanguard, and helping Dr. Shannon with her research in FYRE.
Research Category
Research Proposals
Presentation Type
Poster Presentation
Document Type
Poster
Location
Innovation Forum - 1st Floor Innovation Lab
Presentation Date
10 April 2024, 1:00 pm - 4:00 pm
How does the C-terminus of Iqg1 work in cytokinesis?
Innovation Forum - 1st Floor Innovation Lab
During cytokinesis, the division of the cytoplasm between cells is controlled by the contraction of an actomyosin ring. Iqg1, a crucial regulator of cytokinesis in Saccharomyces cerevisiae, is needed for the assembly and contraction of this ring. Already, it has been found that a domain in the Iqg1 C-terminus is crucial for S. cerevisiae survival. In this study, a regulated Iqg1 allele is introduced to yeast. This will allow researchers to turn off the wild-type allele of the Iqg1 gene under specified conditions, which allows for the study of the effects of the C-terminal Iqg1 deletion. Progress towards making the desired yeast strain is monitored by making protein extracts to examine Iqg1 expression. This research into Iqg1 could potentially provide insight into public health, as IQGAP proteins, a family in which Iqg1 is a member, have implications in human cancer.
