Department
Chemical and Biochemical Engineering
Major
Chemical Engineering with Biochemical emphasis
Research Advisor
Yang, Hu
Advisor's Department
Chemical and Biochemical Engineering
Funding Source
Dr. Yang's Lab
Abstract
In this project ionizable lipids were analyzed for their efficacy in delivery of mRNA in multiple metastatic lung cancer cells. Different ionizable lipids with their molar ratio in making the LNPs were analyzed in order to enhance the nucleic acid delivery to cytoplasm. Phospholipids also play a role in targeting efficacy of the LNPs to the lung cells. Different combination of ionizable lipids with multiple phospholipids were studied for physical characterization and in-vitro transcription efficiency. Transcription efficiency was then quantified using flow cytometry and optimal particles for transcription were noted.
Biography
Noah Sparks is a senior Chemical engineering student with a passion for research. He has worked in Dr. Yang's lab since freshman year and had OURE projects with them for the past two years. He is a hardworking student who works to maintain good grades, while getting experience within the lab. He hopes to work in the biochemical research field and believes undergraduate research will give him the experience necessary to thrive in the field.
Research Category
Engineering
Presentation Type
Poster Presentation
Document Type
Poster
Award
Engineering Poster Session - Third Place
Location
Innovation Forum - 1st Floor Innovation Lab
Presentation Date
10 April 2024, 1:00 pm - 4:00 pm
Ionizable Lipid Nanoparticles Fabrication for Gene Delivery
Innovation Forum - 1st Floor Innovation Lab
In this project ionizable lipids were analyzed for their efficacy in delivery of mRNA in multiple metastatic lung cancer cells. Different ionizable lipids with their molar ratio in making the LNPs were analyzed in order to enhance the nucleic acid delivery to cytoplasm. Phospholipids also play a role in targeting efficacy of the LNPs to the lung cells. Different combination of ionizable lipids with multiple phospholipids were studied for physical characterization and in-vitro transcription efficiency. Transcription efficiency was then quantified using flow cytometry and optimal particles for transcription were noted.
