Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Krishnan, Niranjana
Advisor's Department
Biological Sciences
Funding Source
Missouri S&T
Abstract
Soybean loopers (Chrysodeixis includens) and corn earworms (Helicoverpa zea) are moth pests of soybean and corn fields across the United States, including Missouri. Here, we assessed their toxic and metabolic responses to cypermethrin, a pyrethroid insecticide registered for foliar use in agriculture.
An analysis of literature studies and generation of preliminary dose-response curves in the laboratory indicated that, although corn earworms and soybean loopers belong to the same lepidopteran family (Noctuidae), they show different susceptibilities to topically applied cypermethrin. To assess if metabolic factors are responsible for these differences, we undertook synergistic studies with PBO, TPP, and DEM, which are the inhibitors of three major detoxifying enzymes in the insect body: cytochrome P450s, esterases, and glutathione-s-transferases. We also measured the activity of the detoxifying enzymes through standardized assays.
Findings from this research will be incorporated into a larger project with the goal of identifying surrogate (pest and non-target) species for testing and elucidating differences in susceptibilities. This is necessary to accurately estimate toxic responses within and across taxonomic orders.
Biography
John Sneed is a senior transfer student at Missouri University of Science and Technology majoring in Biological Sciences with a Pre-Medicine emphasis and a minor in Chemistry. He is also completing his associates degree in Liberal Arts at Austin Peay State University in Clarksville, TN. At Missouri S&T, he is the Vice President of National Society of Leadership and Success, the Secretary of Phi Sigma Biological Honor Society, a Representative of Student Council, a member of the Science Olympiad Alumni chapter, and a member of SCRUBS. Before transferring from APSU, he was the Social Enrichment Officer at APSU's chapter of the Alpha Lambda Delta Honor Society. His academic interests include biochemistry, toxicology, and microbiology.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Award
Sciences – section 2 poster presentation, Second place
Location
Upper Atrium
Presentation Date
14 Apr 2022, 1:30 pm - 3:00 pm
Insecticide Metabolic Responses of Two Lepidoptera
Upper Atrium
Soybean loopers (Chrysodeixis includens) and corn earworms (Helicoverpa zea) are moth pests of soybean and corn fields across the United States, including Missouri. Here, we assessed their toxic and metabolic responses to cypermethrin, a pyrethroid insecticide registered for foliar use in agriculture.
An analysis of literature studies and generation of preliminary dose-response curves in the laboratory indicated that, although corn earworms and soybean loopers belong to the same lepidopteran family (Noctuidae), they show different susceptibilities to topically applied cypermethrin. To assess if metabolic factors are responsible for these differences, we undertook synergistic studies with PBO, TPP, and DEM, which are the inhibitors of three major detoxifying enzymes in the insect body: cytochrome P450s, esterases, and glutathione-s-transferases. We also measured the activity of the detoxifying enzymes through standardized assays.
Findings from this research will be incorporated into a larger project with the goal of identifying surrogate (pest and non-target) species for testing and elucidating differences in susceptibilities. This is necessary to accurately estimate toxic responses within and across taxonomic orders.
Comments
Joint project with Amberly Scott and Kathryn Zychinski