Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
OURE
Abstract
During cytokinesis a ring containing actin and myosin forms and contracts, pinching the cell into two daughter cells. In budding yeast cells the gene Iqg1 is a gene necessary for proper actin ring formation and contraction. We have shown that Iqg1 interacts with the yeast formin proteins. This project will determine which domains of Iqg1 mediate the protein-protein interactions, which is important to understand the function of the binding between Iqg1 and formins in cytokinesis. We will use a GST pull down assay to study the binding of Iqg1 to the yeast formins Bni1 and Bnr1. We will compare binding of formins to full length Iqg1 to the ability of formins to bind deletion mutants of Iqg1. The expected outcome is that using the assay, we will identify which region or regions of the Iqg1 protein are required for binding to the formins. This area of research is important for new therapeutic targets in cancer treatment.
Biography
Bethany Huinker is a sophomore in Biological Sciences at Missouri S&T. She is from St. Louis, Missouri. She is involved in the genetic engineering design team on campus. After graduation, Bethany is interested in working in genetic research on familial disorders.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Presentation Date
28 Apr 2017, 10:00 am - 10:15 am
Included in
Protein-protein interaction important for cytokinesis
During cytokinesis a ring containing actin and myosin forms and contracts, pinching the cell into two daughter cells. In budding yeast cells the gene Iqg1 is a gene necessary for proper actin ring formation and contraction. We have shown that Iqg1 interacts with the yeast formin proteins. This project will determine which domains of Iqg1 mediate the protein-protein interactions, which is important to understand the function of the binding between Iqg1 and formins in cytokinesis. We will use a GST pull down assay to study the binding of Iqg1 to the yeast formins Bni1 and Bnr1. We will compare binding of formins to full length Iqg1 to the ability of formins to bind deletion mutants of Iqg1. The expected outcome is that using the assay, we will identify which region or regions of the Iqg1 protein are required for binding to the formins. This area of research is important for new therapeutic targets in cancer treatment.
