Advancement of the Biosynthetic Plant Registry
Department
Chemical and Biochemical Engineering
Major
Chemical Engineering (Biochemical Engineering Emphasis)
Research Advisor
Westenberg, David J.
Advisor's Department
Biological Sciences
Funding Source
Dr. David Westerberg and MS&T IGEM Design Team
Abstract
The 2018 International Genetically Engineered Machines (iGEM) Jamboree conference proved to show flaws in the international plant registry. Although the iGEM registry has over twenty thousand documented parts, only a small percentage make up the plant registry section. This research focused on adding known promoters to the registry to improve future use and documentation. The AT5G54000 promoter is a seed-specific promoter, therefore any gene put after the promoter will affect only the seed. The GmPRP2 promoter is a root-specific promoter. To test the promoters activity, it was combined with the GUS reporter which produces a blue coloration in the specific tissue when given X-gluc. Each promoter's effects can be properly documented. By putting the combination into the specific iGEM backbone, it can be added to the iGEM plant registry to make future projects less time consuming.
Biography
Mikayla is a junior in Chemical Engineering that has done undergraduate research in the past with Dr. Westenberg at MS&T and the National Science Foundation at the University of Houston. She is involved on campus as the secretary of Omega Chi Epsilon, the chemical engineering honor society. Additionally, she participates with the iGEM design team and Chi Omega greek sorority.This upcoming summer (2019) she will be interning with Bayer to do research on plant pathogens.
Presentation Type
OURE Fellows Final Oral Presentation
Document Type
Presentation
Location
Missouri Room
Presentation Date
16 Apr 2019, 10:30 am - 11:00 am
Advancement of the Biosynthetic Plant Registry
Missouri Room
The 2018 International Genetically Engineered Machines (iGEM) Jamboree conference proved to show flaws in the international plant registry. Although the iGEM registry has over twenty thousand documented parts, only a small percentage make up the plant registry section. This research focused on adding known promoters to the registry to improve future use and documentation. The AT5G54000 promoter is a seed-specific promoter, therefore any gene put after the promoter will affect only the seed. The GmPRP2 promoter is a root-specific promoter. To test the promoters activity, it was combined with the GUS reporter which produces a blue coloration in the specific tissue when given X-gluc. Each promoter's effects can be properly documented. By putting the combination into the specific iGEM backbone, it can be added to the iGEM plant registry to make future projects less time consuming.
