MP3: Microparticle-laden Protein Particles for Wound Healing
Department
Chemical and Biochemical Engineering
Major
Chemical Engineering
Research Advisor
Barua, Sutapa
Advisor's Department
Chemical and Biochemical Engineering
Funding Source
University of Missouri Research Board
Abstract
The objective of this research is to synthesize biodegradable microparticles (MP3s) to serve as a structural matrix for human cells, with the end goal of application for burn wound healing. The MP3s will be synthesized using poly(lactic-co-glycolic acid) (PLGA) polymer using the single emulsion solvent-evaporation technique while incorporating a proprietary flowfocusing device. Fibronectin will be immobilized on the surface of MP3s using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and Nhydroxysuccinimide (NHS) conjugation and quantified using the bicinchoninic acid (BCA) assay. The conjugated MP3s will be seeded with human umbilical vein endothelial cells (HUVECs) and assessed for cell binding ability. The viable cell attachment and proliferation will be qualitatively analyzed using fluorescent microscopy. It is expected that fibronectin immobilization on the surface of MP3s will increase cell attachment compared to unmodified particles or particles coated with other proteins. The future of this project will be the study of effects of MP3s for tissue regeneration.
Biography
Elizabeth Lemieux is a sophomore in Chemical Engineering at Missouri S&T, pursuing minors in Chemistry and Nuclear Engineering. She is a member of Chi Omega Fraternity, Society of Women Engineers, and is on the executive board for Engineers Without Borders. Elizabeth hopes to graduate in May 2020.
Research Category
Engineering
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium
Presentation Date
17 Apr 2018, 1:00 pm - 4:00 pm
MP3: Microparticle-laden Protein Particles for Wound Healing
Upper Atrium
The objective of this research is to synthesize biodegradable microparticles (MP3s) to serve as a structural matrix for human cells, with the end goal of application for burn wound healing. The MP3s will be synthesized using poly(lactic-co-glycolic acid) (PLGA) polymer using the single emulsion solvent-evaporation technique while incorporating a proprietary flowfocusing device. Fibronectin will be immobilized on the surface of MP3s using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and Nhydroxysuccinimide (NHS) conjugation and quantified using the bicinchoninic acid (BCA) assay. The conjugated MP3s will be seeded with human umbilical vein endothelial cells (HUVECs) and assessed for cell binding ability. The viable cell attachment and proliferation will be qualitatively analyzed using fluorescent microscopy. It is expected that fibronectin immobilization on the surface of MP3s will increase cell attachment compared to unmodified particles or particles coated with other proteins. The future of this project will be the study of effects of MP3s for tissue regeneration.
