Investigating the function of the IQG1 domain in yeast cytokinesis
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
Opportunities for Undergraduate Research Experiences (OURE)
Abstract
The budding yeast protein IQG1 is required for both actomyosin ring assembly and contraction during cytokinesis. Iqg1 interacts with two other proteins required for actin ring formation, the formins Bni1 and Bnr1. In order to further investigate the function of the interaction between Iqg1 and the formins, the first step is to identify which Iqg1 protein domain is required for the interaction. My project will be to investigate the binding using mutant strains lacking different Iqg1 domains. I will be preparing yeast protein extracts and performing protein binding analysis using GST pull down assays and Western blotting. Once the functional domain of Iqg1 is identified, we can further investigate the role of the Iqg1-formin interaction by doing a phenotypic analysis of Iqg1 mutants that cannot bind to formins.
Biography
Lindsey Pratt is a junior here at Missouri University of Science and Technology. She is majoring in biological sciences and minoring in chemistry. Lindsey belongs to several organizations including; Scrubs, National Society of Leadership and Success and the Honor Society. She works part time as a scribe for PhysAssist Scribes. She lives on a large cattle farm and enjoys spending most of her days outside. She hopes to be accepted to Mizzou’s medical program so that she can return home and practice as a general physician.
Research Category
Research Proposals
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
11 Apr 2016, 9:00 am - 11:45 am
Investigating the function of the IQG1 domain in yeast cytokinesis
Upper Atrium/Hallway
The budding yeast protein IQG1 is required for both actomyosin ring assembly and contraction during cytokinesis. Iqg1 interacts with two other proteins required for actin ring formation, the formins Bni1 and Bnr1. In order to further investigate the function of the interaction between Iqg1 and the formins, the first step is to identify which Iqg1 protein domain is required for the interaction. My project will be to investigate the binding using mutant strains lacking different Iqg1 domains. I will be preparing yeast protein extracts and performing protein binding analysis using GST pull down assays and Western blotting. Once the functional domain of Iqg1 is identified, we can further investigate the role of the Iqg1-formin interaction by doing a phenotypic analysis of Iqg1 mutants that cannot bind to formins.
