Phenotype Comparison of two Different IQG1 Mutant Alleles
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Abstract
During cytokinesis, many mutations can arise that affect formation of the actomyosin ring. Iqg1 is a protein that is required for assembly and contraction of the actomyosin ring in budding yeast. This project is designed to compare the phenotypes of two separate IQG1 mutant alleles to examine any problems that arise during cytokinesis. In this research, a mutant (3A) that has three serines dephosphorylated via CDC14 mutated to alanine is compared to a mutant (4A) that has three serines and one threonine mutated to alanine. The goal in doing this is to compare cytokinesis defects in the 3A mutant and the 4A mutant and see if threonine has a unique function. To confirm mutant phenotype, morphological analysis will be performed via microscopy and immunofluorescence to indicate if actin ring formation has been disrupted.
Biography
Madison Mara is a freshman majoring in biological sciences and plans to enter medical school at the end of her undergrad. She has been working in Dr.Shannon’s cytokinesis lab since June 2014 and this represents her first project. She is currently the Community Chair on the Executive Committee for Scrubs and a member of Helix.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hall
Presentation Date
15 Apr 2015, 9:00 am - 11:45 am
Phenotype Comparison of two Different IQG1 Mutant Alleles
Upper Atrium/Hall
During cytokinesis, many mutations can arise that affect formation of the actomyosin ring. Iqg1 is a protein that is required for assembly and contraction of the actomyosin ring in budding yeast. This project is designed to compare the phenotypes of two separate IQG1 mutant alleles to examine any problems that arise during cytokinesis. In this research, a mutant (3A) that has three serines dephosphorylated via CDC14 mutated to alanine is compared to a mutant (4A) that has three serines and one threonine mutated to alanine. The goal in doing this is to compare cytokinesis defects in the 3A mutant and the 4A mutant and see if threonine has a unique function. To confirm mutant phenotype, morphological analysis will be performed via microscopy and immunofluorescence to indicate if actin ring formation has been disrupted.
