The use of dynamic light scattering to follow the aggregation of bovine insulin
Department
Chemical and Biochemical Engineering
Major
Biochemical Engineering
Research Advisor
Forciniti, Daniel
Advisor's Department
Chemical and Biochemical Engineering
Abstract
Insulin forms fibrils rich in beta sheets at extreme pHs and temperatures. The fibrils may be identified by a couple of fluorescence assays and visualized by SEM. Dynamic light scattering is a powerful technique that can be used to monitor in situ the change in size over time of the fibrils. Moreover, information may be obtained about the size distribution and about the shape of the aggregates. In this work, human and bovine insulin were incubated at pH 1.8 in the presence of NaCl and at a temperature of 60 C. The samples were periodically monitored using a dynamic light scattering instrument working at 800 nm and at an angle of 155⁰. Deconvolution of the signals was done to identify the main populations and how they evolve over time. The results were compared with theoretical calculations. Differences between the insulins from both species are highlighted.
Biography
Kirstin Bier is a senior in biochemical engineering researching under Dr. Forciniti.
Research Category
Engineering
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hall
Presentation Date
15 Apr 2015, 1:00 pm - 3:00 pm
The use of dynamic light scattering to follow the aggregation of bovine insulin
Upper Atrium/Hall
Insulin forms fibrils rich in beta sheets at extreme pHs and temperatures. The fibrils may be identified by a couple of fluorescence assays and visualized by SEM. Dynamic light scattering is a powerful technique that can be used to monitor in situ the change in size over time of the fibrils. Moreover, information may be obtained about the size distribution and about the shape of the aggregates. In this work, human and bovine insulin were incubated at pH 1.8 in the presence of NaCl and at a temperature of 60 C. The samples were periodically monitored using a dynamic light scattering instrument working at 800 nm and at an angle of 155⁰. Deconvolution of the signals was done to identify the main populations and how they evolve over time. The results were compared with theoretical calculations. Differences between the insulins from both species are highlighted.
