Title

Analysis of the Second Virial Coefficient of Glycosylated Proteins by Light Scattering

Presenter Information

Sam Ruesing

Department

Chemical and Biochemical Engineering

Major

Chemical Engineering

Research Advisor

Forciniti, Daniel

Advisor's Department

Chemical and Biochemical Engineering

Funding Source

Missouri S& T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

The second virial coefficient, A2, has been used by others to characterize the stability of a protein in solution to screen for proper crystallization conditions. However, the effect of protein glycosylation on A2 has not been investigated. Ribonuclease A and B are isoforms of the same protein that differ by a single glycosylation. Solutions of Ribonuclease A were analyzed by light scattering at two pH and temperature levels. A2 was then determined for each condition by Zimm Plot analysis. Ribonuclease B was not tested yet. In future studies, A2 for Ribonuclease B will be measured and compared to the determined values of Ribonuclease A to determine the effect of glycosylation patterns on A2.

Biography

Sam is from Pontoon Beach, IL, is a senior in Chemical Engineering. He is the Vice-President of Missouri S& T's Academic Competition Organization and staffs high school Scholar Bowl tournaments throughout Missouri. He will graduate in May, 2013.

Research Category

Engineering

Presentation Type

Oral Presentation

Document Type

Presentation

Award

Engineering oral presentation, First place

Location

Carver Room

Presentation Date

03 Apr 2013, 10:30 am - 11:00 am

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Apr 3rd, 10:30 AM Apr 3rd, 11:00 AM

Analysis of the Second Virial Coefficient of Glycosylated Proteins by Light Scattering

Carver Room

The second virial coefficient, A2, has been used by others to characterize the stability of a protein in solution to screen for proper crystallization conditions. However, the effect of protein glycosylation on A2 has not been investigated. Ribonuclease A and B are isoforms of the same protein that differ by a single glycosylation. Solutions of Ribonuclease A were analyzed by light scattering at two pH and temperature levels. A2 was then determined for each condition by Zimm Plot analysis. Ribonuclease B was not tested yet. In future studies, A2 for Ribonuclease B will be measured and compared to the determined values of Ribonuclease A to determine the effect of glycosylation patterns on A2.