Comparison of Potency of SOCE Inhibition by Naturally Occurring Biphenolic Compounds
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Aronstam, Robert
Advisor's Department
Biological Sciences
Funding Source
cDNA Resource Center
Abstract
Asian herbal medicine has used plants to treat many diseases for years. Two main compounds derived from Magnolia trees, honokiol and magnolol, have become accepted in their treatment of mental disorders such as anxiety and depression. These compounds have more recently been studied for their unique abilities to induce apoptosis and cytotoxicity in cancer cell lines. The purpose of this study was to determine and compare how honokiol, its sister compound, magnolol, and two other isomers, compound 24 and 27, affect intracellular calcium concentrations in cells expressing muscarinic receptors. Calcium concentrations were determined using a Fura-2 ratiometric imaging protocol. It was determined that all four compounds inhibit SOCE, an ER calcium-replenishing mechanism, known to play a crucial role in apoptosis and cell cycle regulation. Of this, compound 27 was found to be the most potent. It is recommended that the isomers of honokiol be specifically looked at for their anti-tumorigenic properties.
Biography
Alexis is a senior of the Biological Science’s department. She has been a research assistant in the cDNA Resource Center for two and a half years. Alexis is an active member of the Biological Honor’s society, Phi Sigma, and secretary of a women’s service organization, Lambda Sigma Pi. She will be attending medical school at Kansas City University of Medicine and Biosciences in the fall.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
10 Apr 2012, 9:00 am - 11:45 am
Comparison of Potency of SOCE Inhibition by Naturally Occurring Biphenolic Compounds
Upper Atrium/Hallway
Asian herbal medicine has used plants to treat many diseases for years. Two main compounds derived from Magnolia trees, honokiol and magnolol, have become accepted in their treatment of mental disorders such as anxiety and depression. These compounds have more recently been studied for their unique abilities to induce apoptosis and cytotoxicity in cancer cell lines. The purpose of this study was to determine and compare how honokiol, its sister compound, magnolol, and two other isomers, compound 24 and 27, affect intracellular calcium concentrations in cells expressing muscarinic receptors. Calcium concentrations were determined using a Fura-2 ratiometric imaging protocol. It was determined that all four compounds inhibit SOCE, an ER calcium-replenishing mechanism, known to play a crucial role in apoptosis and cell cycle regulation. Of this, compound 27 was found to be the most potent. It is recommended that the isomers of honokiol be specifically looked at for their anti-tumorigenic properties.
