Is IQG1 required for the localization of Bni1?
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Abstract
Saccharomyces cerevisiae, budding yeast, is our model organism to study cytokinesis. Cytokinesis is an important and essential step of cell division. During cytokinesis, the cell separates the cytoplasm through the contraction of F-actin and non muscle type II myosin.
udding yeast contain two formins, Bni1 and Bnr1. These are essential for cytokinesis. My hypothesis focuses on IQG1 and Bni1. My project focuses on if IQG1 is required for the localization Bni1 at the site of cytokinesis.
n order to determine if Iqg1 is required for the localization of Bni1, I must observe the bud neck localization of Bni1 using GFP. My goal is to compare the Bni1 neck localization with and without IqgI. Bni1-GFP is observed in mitotic cells. Galactose media allows expression of Iqg1, while glucose represses Iqg1 expression. Data indicate that Bni1-GFP does localize without Iqg1; quantification will be performed to measure Bni1-GFP at the bud neck.
Biography
Mydah is a senior in Biological Sciences at the Missouri University of Science and Technology. She started working with Dr. Katie Shannon in August 2011. Mydah is an officer in SCRUBS Pre Health Club as well as an active member in Phi Sigma Biological Sciences Honors Society and Helix Life Sciences Club. Mydah plans to attend medical school post graduation from Missouri University of Science and Technology.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
10 Apr 2012, 9:00 am - 11:45 am
Is IQG1 required for the localization of Bni1?
Upper Atrium/Hallway
Saccharomyces cerevisiae, budding yeast, is our model organism to study cytokinesis. Cytokinesis is an important and essential step of cell division. During cytokinesis, the cell separates the cytoplasm through the contraction of F-actin and non muscle type II myosin.
udding yeast contain two formins, Bni1 and Bnr1. These are essential for cytokinesis. My hypothesis focuses on IQG1 and Bni1. My project focuses on if IQG1 is required for the localization Bni1 at the site of cytokinesis.
n order to determine if Iqg1 is required for the localization of Bni1, I must observe the bud neck localization of Bni1 using GFP. My goal is to compare the Bni1 neck localization with and without IqgI. Bni1-GFP is observed in mitotic cells. Galactose media allows expression of Iqg1, while glucose represses Iqg1 expression. Data indicate that Bni1-GFP does localize without Iqg1; quantification will be performed to measure Bni1-GFP at the bud neck.
