Effects of Chlorothiazide on Muscarinic Receptors
Department
Biological Sciences
Major
Biology
Research Advisor
Aronstam, Robert
Advisor's Department
Biological Sciences
Funding Source
cDNA Resource lab
Abstract
Chlorothiazide (6-chloro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide) is diuretic drug normally used within a hospital setting to manage excess fluid associated with congestive heart failure and as an antihypertensive drug. The goal of this experiment was to define the effects of Chlorothiazide on muscarinic receptors. We determined the effects of Chlorothiazide on calcium signaling by using ratiometric fluorescent dye on Chinese hamster ovary (CHO) cells, which have the human M3 muscarinic receptors, and imaging the amounts of intracellular calcium concentrations released. Based on the results of the experiment conducted, Chlorothiazide has no legitimate inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. Chlorothiazide most likely acts as a stimulant to the uptake of calcium.
Biography
Katie is a sophomore biological sciences student at Missouri University of Science and Technology. Katie has volunteered as a student researcher for eleven months in the cDNA research center and greatly enjoyed the research opportunities introduced to her by volunteering in the lab.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
10 Apr 2012, 9:00 am - 11:45 am
Effects of Chlorothiazide on Muscarinic Receptors
Upper Atrium/Hallway
Chlorothiazide (6-chloro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide) is diuretic drug normally used within a hospital setting to manage excess fluid associated with congestive heart failure and as an antihypertensive drug. The goal of this experiment was to define the effects of Chlorothiazide on muscarinic receptors. We determined the effects of Chlorothiazide on calcium signaling by using ratiometric fluorescent dye on Chinese hamster ovary (CHO) cells, which have the human M3 muscarinic receptors, and imaging the amounts of intracellular calcium concentrations released. Based on the results of the experiment conducted, Chlorothiazide has no legitimate inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. Chlorothiazide most likely acts as a stimulant to the uptake of calcium.
Comments
Joint project with Brittany Brand