Title

Determining the interaction between IQG1 and formin proteins

Presenter Information

Rachel Wille

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Shannon, Katie

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

Our lab focuses on the study of cytokinesis in Saccharomyces cerevisiae (budding yeast). Cytokinesis is the separation of a cell into two daughter cells. My current research focuses on the interaction between two protein formins, Bni1 and Bnr1, and the IQG1 protein. It is believed that there is an interaction between these formins and the IQG1 complex because in C.albicans, another yeast species, the IQG1 homolog interacted with formins. In budding yeast, IQG1 and the formins are required for actin ring formation. At this time I am still trying to determine the interaction between the protein formins and IQG1. Co-immunoprecipitation experiments did not show an interaction. However, it is likely that the interaction occurs, but is regulated. Currently, we are working with new plasmid that contains a domain that possibly regulates this potential interaction. Using this DAD domain, we hope to detect the interaction between the formins and IQG1.

Biography

Rachel is a graduating senior at Missouri University of Science and Technology. She is a Biological Science major and a member of the Women’s Soccer team and Phi Sigma. Rachel also has an OURE in the cytokinesis lab this year. Upon graduation Rachel plans to apply to graduate schools and obtain her Ph.D.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Location

Upper Atrium/Hallway

Presentation Date

06 Apr 2011, 9:00 am - 11:45 am

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Apr 6th, 9:00 AM Apr 6th, 11:45 AM

Determining the interaction between IQG1 and formin proteins

Upper Atrium/Hallway

Our lab focuses on the study of cytokinesis in Saccharomyces cerevisiae (budding yeast). Cytokinesis is the separation of a cell into two daughter cells. My current research focuses on the interaction between two protein formins, Bni1 and Bnr1, and the IQG1 protein. It is believed that there is an interaction between these formins and the IQG1 complex because in C.albicans, another yeast species, the IQG1 homolog interacted with formins. In budding yeast, IQG1 and the formins are required for actin ring formation. At this time I am still trying to determine the interaction between the protein formins and IQG1. Co-immunoprecipitation experiments did not show an interaction. However, it is likely that the interaction occurs, but is regulated. Currently, we are working with new plasmid that contains a domain that possibly regulates this potential interaction. Using this DAD domain, we hope to detect the interaction between the formins and IQG1.