Determining the interaction between IQG1 and formin proteins
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Our lab focuses on the study of cytokinesis in Saccharomyces cerevisiae (budding yeast). Cytokinesis is the separation of a cell into two daughter cells. My current research focuses on the interaction between two protein formins, Bni1 and Bnr1, and the IQG1 protein. It is believed that there is an interaction between these formins and the IQG1 complex because in C.albicans, another yeast species, the IQG1 homolog interacted with formins. In budding yeast, IQG1 and the formins are required for actin ring formation. At this time I am still trying to determine the interaction between the protein formins and IQG1. Co-immunoprecipitation experiments did not show an interaction. However, it is likely that the interaction occurs, but is regulated. Currently, we are working with new plasmid that contains a domain that possibly regulates this potential interaction. Using this DAD domain, we hope to detect the interaction between the formins and IQG1.
Biography
Rachel is a graduating senior at Missouri University of Science and Technology. She is a Biological Science major and a member of the Women’s Soccer team and Phi Sigma. Rachel also has an OURE in the cytokinesis lab this year. Upon graduation Rachel plans to apply to graduate schools and obtain her Ph.D.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
06 Apr 2011, 9:00 am - 11:45 am
Determining the interaction between IQG1 and formin proteins
Upper Atrium/Hallway
Our lab focuses on the study of cytokinesis in Saccharomyces cerevisiae (budding yeast). Cytokinesis is the separation of a cell into two daughter cells. My current research focuses on the interaction between two protein formins, Bni1 and Bnr1, and the IQG1 protein. It is believed that there is an interaction between these formins and the IQG1 complex because in C.albicans, another yeast species, the IQG1 homolog interacted with formins. In budding yeast, IQG1 and the formins are required for actin ring formation. At this time I am still trying to determine the interaction between the protein formins and IQG1. Co-immunoprecipitation experiments did not show an interaction. However, it is likely that the interaction occurs, but is regulated. Currently, we are working with new plasmid that contains a domain that possibly regulates this potential interaction. Using this DAD domain, we hope to detect the interaction between the formins and IQG1.
