Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Aronstam, Robert
Advisor's Department
Biological Sciences
Funding Source
Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.
Biography
Alexis is a junior from Rogersville, MO, planning to attend medical school after receiving her bachelor’s degree in Biological Sciences. Alexis is an active member in Phi Sigma, the Biological Sciences honor society, as well as the other Biological Sciences organizations, Scrubs and Helix. Alexis is employed as a research assistant in Dr. Aronstam’s laboratory, conducting research on signal transduction in cells. Alexis has been awarded the Academic Scholar’s certification for all three years of undergraduate studies. In her free time, she mentors elementary children, and volunteers at St. John’s Free Clinic, as well as other various local charities. At the end of March, Alexis will be presenting additional research at the American Society for Neurochemistry conference in St. Louis, MO.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Award
Sciences poster session, Second place
Location
Upper Atrium/Hallway
Presentation Date
06 Apr 2011, 9:00 am - 11:45 am
Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor
Upper Atrium/Hallway
Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.
Comments
Joint project with Megan Koerner