Title

Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor

Presenter Information

Alexis Martin

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.

Biography

Alexis is a junior from Rogersville, MO, planning to attend medical school after receiving her bachelor’s degree in Biological Sciences. Alexis is an active member in Phi Sigma, the Biological Sciences honor society, as well as the other Biological Sciences organizations, Scrubs and Helix. Alexis is employed as a research assistant in Dr. Aronstam’s laboratory, conducting research on signal transduction in cells. Alexis has been awarded the Academic Scholar’s certification for all three years of undergraduate studies. In her free time, she mentors elementary children, and volunteers at St. John’s Free Clinic, as well as other various local charities. At the end of March, Alexis will be presenting additional research at the American Society for Neurochemistry conference in St. Louis, MO.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Award

Sciences poster session, Second place

Location

Upper Atrium/Hallway

Presentation Date

06 Apr 2011, 9:00 am - 11:45 am

Comments

Joint project with Megan Koerner

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Apr 6th, 9:00 AM Apr 6th, 11:45 AM

Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor

Upper Atrium/Hallway

Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.