Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Aronstam, Robert
Advisor's Department
Biological Sciences
Funding Source
Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.
Biography
Megan is in her third year at Missouri S&T. She is from Eldon, MO, and plans to attend medical school after receiving her undergraduate degree in Biological Sciences. She will receive a minor in Cognitive Neuroscience and Chemistry. Megan is an active member in the Biological Sciences Honor Society. Other campus organizations she is involved in include Scrubs, Helix, and Engineers Without Borders. Megan is employed as a department recruiter for the Biological Sciences Department. She also volunteers in Dr. Aronstam’s laboratory, conducting research on cellular signal transduction. Megan has been awarded the Access Missouri Scholarship and SMART Grant all three years of academic study.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Award
Sciences poster session, Second place
Location
Upper Atrium/Hallway
Presentation Date
06 Apr 2011, 9:00 am - 11:45 am
Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor
Upper Atrium/Hallway
Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.
Comments
Joint project with Alexis Martin