Title

Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor

Presenter Information

Megan Koerner

Department

Biological Sciences

Major

Biological Sciences

Research Advisor

Aronstam, Robert

Advisor's Department

Biological Sciences

Funding Source

Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program

Abstract

Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.

Biography

Megan is in her third year at Missouri S&T. She is from Eldon, MO, and plans to attend medical school after receiving her undergraduate degree in Biological Sciences. She will receive a minor in Cognitive Neuroscience and Chemistry. Megan is an active member in the Biological Sciences Honor Society. Other campus organizations she is involved in include Scrubs, Helix, and Engineers Without Borders. Megan is employed as a department recruiter for the Biological Sciences Department. She also volunteers in Dr. Aronstam’s laboratory, conducting research on cellular signal transduction. Megan has been awarded the Access Missouri Scholarship and SMART Grant all three years of academic study.

Research Category

Sciences

Presentation Type

Poster Presentation

Document Type

Poster

Award

Sciences poster session, Second place

Location

Upper Atrium/Hallway

Presentation Date

06 Apr 2011, 9:00 am - 11:45 am

Comments

Joint project with Alexis Martin

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Apr 6th, 9:00 AM Apr 6th, 11:45 AM

Honokiol Blocks Store Operated Calcium Entry in CHO Cells Expressing the M3 Muscarinic Receptor

Upper Atrium/Hallway

Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees, inhibits multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission mediated by M3 muscarinic receptors expressed in Chinese hamster ovary (CHO) cells. Honokiol had a potent (EC50 ≈ 5 μM) inhibitory effect on store operated calcium entry (SOCE) that was induced by activation of the M3 receptors. This effect was specific, rapid and reversible, and was seen at concentrations not associated with cytotoxicity, inhibition of IP3 receptor-mediated calcium release, depletion of ER calcium stores, or disruption of M3 receptor binding. It is likely that an inhibition of SOCE contributes to honokiol disruption of parasympathetic motor functions, as well as many of its beneficial pharmacological properties.