Regulation of Cytokinesis in Budding Yeast by Kinase Dbf2
Department
Biological Sciences
Major
Biological Sciences
Research Advisor
Shannon, Katie
Advisor's Department
Biological Sciences
Funding Source
Missouri S&T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
This research focuses on cytokinesis in budding yeast, specifically how Dbf2 is recruited to the bud neck of a cell undergoing cytokinesis and if the kinase activity of Dbf2 is required for cytokinesis. Dbf2 is a kinase in the mitotic exit network (MEN) that moves from the spindle pole body to the bud neck in late mitosis in order to be in position to possibly regulate actomyosin ring contraction. The main question for this research was whether or not Dbf2 could localize with no actin ring present during cytokinesis. Dbf2 was tagged with the HA epitope in a yeast strain in which actin ring formation is prevented by addition of glucose. Immunofluorescence of cells under control conditions and when no actin ring is formed showed that Dbf2 does not require actin to localize to the bud neck. It was found that a dbf2-2 dbf20Δ with a kinase-dead mutant Dbf2 allele strain exhibited cytokinesis defects at the non-permissive temperature, demonstrating that Dbf2 kinase activity is required for cytokinesis.
Biography
Brittany Hood is a senior at Missouri University of Science and Technology majoring in Biological Sciences. Brittany is from St. Louis, MO. She plans on continuing her education after graduation to obtain a Bachelor’s of Science in Nursing. She coaches and plays club volleyball, and is a member of a local youth group.
Research Category
Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Location
Upper Atrium/Hallway
Presentation Date
07 Apr 2010, 9:00 am - 11:45 am
Regulation of Cytokinesis in Budding Yeast by Kinase Dbf2
Upper Atrium/Hallway
This research focuses on cytokinesis in budding yeast, specifically how Dbf2 is recruited to the bud neck of a cell undergoing cytokinesis and if the kinase activity of Dbf2 is required for cytokinesis. Dbf2 is a kinase in the mitotic exit network (MEN) that moves from the spindle pole body to the bud neck in late mitosis in order to be in position to possibly regulate actomyosin ring contraction. The main question for this research was whether or not Dbf2 could localize with no actin ring present during cytokinesis. Dbf2 was tagged with the HA epitope in a yeast strain in which actin ring formation is prevented by addition of glucose. Immunofluorescence of cells under control conditions and when no actin ring is formed showed that Dbf2 does not require actin to localize to the bud neck. It was found that a dbf2-2 dbf20Δ with a kinase-dead mutant Dbf2 allele strain exhibited cytokinesis defects at the non-permissive temperature, demonstrating that Dbf2 kinase activity is required for cytokinesis.
