Department
Chemical and Biochemical Engineering
Major
Chemical Engineering
Research Advisor
Brown, Roger F.
Advisor's Department
Biological Sciences
Funding Source
Missouri S& T Opportunities for Undergraduate Research Experiences (OURE) Program
Abstract
Two types of glass scaffolds fabricated from silicate-based 13-93 glass were tested for possible use in bone tissue engineering. The two scaffold types included constructs prepared by a polymer foam infiltration procedure to simulate trabecular bone and sintered glass fiber construct. These scaffolds were seeded with mouse MLO-AS cells, an established line of osteogenic cells used as a model of developing bone tissue. Assays were conducted to assess growth and function of the seeded cells over incubation times ranging from two to six days. Cell adhesion to the scaffolds was confirmed by scanning electron microscopy. Quantitative measurements of total cellular protein indicate that the rate of cell growth on the scaffolds is similar to that on standard tissue culture plates. MTT assays showed significant penetration and proliferation of cells within the interior of the scaffolds. Alkaline phosphatase, a marker enzyme of bone cell differentiation, was assayed to assess cell function of the scaffolds. Alkaline activity of this marker enzyme increased linearly during the six-day incubation on both scaffolds indicating they both support differentiation. The results obtained suggest that 13-93 sponge and fiber scaffolds are effective frameworks for bone tissue engineering.
Biography
Kevin Walker is a junior at the Missouri University of Science and Technology. He is majoring in Chemical Engineering with a minor in Biological Sciences. Kevin is a recipient of the Missouri S& T Chancellor's Scholarship. He serves as the president of the Christian Campus Fellowship and as a Resident Assistant at Missouri S& T. After graduation Kevin plans to pursue a Doctor of Medicine degree.
Research Category
Natural Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Award
Natural Sciences poster session, Second place
Location
Havener Center, Upper Atrium/Hallway
Presentation Date
9 Apr 2008, 1:00 pm - 3:00 pm
In Vitro Assessment of Porous Glass Scaffolds for Bone Tissue Engineering
Havener Center, Upper Atrium/Hallway
Two types of glass scaffolds fabricated from silicate-based 13-93 glass were tested for possible use in bone tissue engineering. The two scaffold types included constructs prepared by a polymer foam infiltration procedure to simulate trabecular bone and sintered glass fiber construct. These scaffolds were seeded with mouse MLO-AS cells, an established line of osteogenic cells used as a model of developing bone tissue. Assays were conducted to assess growth and function of the seeded cells over incubation times ranging from two to six days. Cell adhesion to the scaffolds was confirmed by scanning electron microscopy. Quantitative measurements of total cellular protein indicate that the rate of cell growth on the scaffolds is similar to that on standard tissue culture plates. MTT assays showed significant penetration and proliferation of cells within the interior of the scaffolds. Alkaline phosphatase, a marker enzyme of bone cell differentiation, was assayed to assess cell function of the scaffolds. Alkaline activity of this marker enzyme increased linearly during the six-day incubation on both scaffolds indicating they both support differentiation. The results obtained suggest that 13-93 sponge and fiber scaffolds are effective frameworks for bone tissue engineering.
