The Growth of MC3T3-E1 Pre-Osteoblasts on Borate Containing Glasses
Department
Biological Sciences
Major
Biological Science
Research Advisor
Brown, Roger F.
Rahaman, M. N., 1950-
Advisor's Department
Biological Sciences
Second Advisor's Department
Materials Science and Engineering
Funding Source
Musculoskeletal Transplant Foundation (Rahaman, PI)
Abstract
Borate-based glasses in the B2O3-SiO2-Na2O-CaO-P2O5 system were recently developed at this institution for possible use as scaffolds in bone tissue engineering. In this study, three of the borate-based glasses, compositions designated 1B, 2B, and 3B, with B2O3 levels of 15, 31, and 46 mole percent, respectively, were tested for their effects on the growth of MC3T3-E1 mouse pre-osteoblastic cells. Silicate type 45S5 glass was used as a control for comparison. Tests performed included: contact assays of cell growth at the glass interface, quantitative fluorescence assay of cell proliferation in the presence of the test glasses, chemical analysis of borate released into the culture medium, and scanning electron microscope imaging. The results obtained suggest that 1B and 2B glasses permit a reasonable level of MC3T3-E1 cell growth and appear promising for possible use in bone tissue engineering.
Biography
Agatha Dwilewicz is a sophomore undergraduate student at the University of Missouri-Rolla, majoring in Biological Sciences.
Research Category
Natural Sciences
Presentation Type
Poster Presentation
Document Type
Poster
Award
Natural Sciences poster session, Second place
Presentation Date
12 Apr 2006, 1:00 pm
The Growth of MC3T3-E1 Pre-Osteoblasts on Borate Containing Glasses
Borate-based glasses in the B2O3-SiO2-Na2O-CaO-P2O5 system were recently developed at this institution for possible use as scaffolds in bone tissue engineering. In this study, three of the borate-based glasses, compositions designated 1B, 2B, and 3B, with B2O3 levels of 15, 31, and 46 mole percent, respectively, were tested for their effects on the growth of MC3T3-E1 mouse pre-osteoblastic cells. Silicate type 45S5 glass was used as a control for comparison. Tests performed included: contact assays of cell growth at the glass interface, quantitative fluorescence assay of cell proliferation in the presence of the test glasses, chemical analysis of borate released into the culture medium, and scanning electron microscope imaging. The results obtained suggest that 1B and 2B glasses permit a reasonable level of MC3T3-E1 cell growth and appear promising for possible use in bone tissue engineering.
