Department
Chemistry
Research Advisor
Ma, Yinfa
Advisor's Department
Chemistry
Abstract
We have continued our development of a biosensor and are developing new methods of antigen detection. A fluorometer was previously constructed and was shown to have the ability of quantitatively detecting the amount of fluorescence of fluorescent beads purchased from Molecular Probes. This ability thus corresponds to an ability to quantitate the amount of fluorescent beads which are in solution. Difficulties arose in attaching protein to these beads by the method given by molecular probes and this problem was examined using UV-Vis spectroscopy. Amongst other proposed approaches to antigen detection, one most attachment of antibody lo gold so that antigen could be detected by bridging the gold and antibody covered beads. An alternative to this which is planned to be examined is to use polymer trays with wells from ELISA kits instead of gold substrate.
Presentation Type
Oral Presentation
Document Type
Presentation
Presentation Date
2004-2005
Recommended Citation
Fennewald, Tom, "Exploration of Various Techniques for Quantitative Detection of Antibodies in Solution" (2004). Opportunities for Undergraduate Research Experience Program (OURE). 235.
https://scholarsmine.mst.edu/oure/235
